In this comparative study two types of temperature transition strategies from mesophilic to thermophilic conditions in anaerobic digestion was explored. Both strategies used a rapid increase from 37 to 55°C with a constant organic loading rate (2.4 kg VS m-3 d-1) and hydraulic retention time (14 d). The two digesters used the same mesophilic inoculoum but in the second digester a small share of thermophilic digeastate was also inoculated. A comparative dewaterability study between the fullscale mesophilic digestate and the thermophilic digestates were also performed as part of the study. The results showed a stabilization in both digesters within 14 days (1 Hydraulic retention time). The digester where a small share of thermophilic inoculum was introduced had a higher methane production compared to the control reactor where just mesophilic inoculum was used. The comparative dewaterability study showed a deterioration of the dewaterability in both digesters when thermophilic conditions was established.