Monitoring and quantification of inclusion body formation in Escherichia coli by multi-parameter flow cytometryShow others and affiliations
2005 (English)In: Biotechnology Letters, ISSN 0141-5492, Vol. 27, no 13, p. 919-926Article in journal (Refereed) Published
Abstract [en]
Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6-48 mg PMP/g CDW) whilst highlighting population heterogeneity.
Place, publisher, year, edition, pages
2005. Vol. 27, no 13, p. 919-926
Keywords [en]
Bioreactors/microbiology, Chromatography; High Pressure Liquid, Escherichia coli/growth & development/*metabolism, Fermentation, Flow Cytometry/*methods, Fluorescent Dyes/chemistry, Humans, Inclusion Bodies/chemistry/*metabolism, Interleukin-3, Microscopy; Fluorescence, Receptors; Interleukin-3/chemistry/genetics/metabolism, Recombinant Fusion Proteins, Recombinant Proteins/metabolism, Thrombopoietin/chemistry/genetics/metabolism
National Category
Biophysics
Identifiers
URN: urn:nbn:se:mdh:diva-2154DOI: 10.1007/s10529-005-7184-6ISI: 000231164000007PubMedID: 16091887Scopus ID: 2-s2.0-23844548756OAI: oai:DiVA.org:mdh-2154DiVA, id: diva2:114817
2006-03-092006-03-092025-10-10Bibliographically approved