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This study evaluated the performance of a newly proposed hybrid ventilation strategy, a temperature-controlled airflow system, in an operating room under different working conditions. The influence of obstacles and ventilation rates on air patterns and airborne contamination concentration is investigated. Numerical simulation based on computational fluid dynamics techniques was applied to obtain high-resolution whole-field flow movement and particle dispersion. The results show that obstacles like surgical lamps considerably weaken the central supply of air and introduce mixing air movement above the operating table. It is also found that due to the presence of surgical lamps, the air exchange rate should be increased to obtain a similar performance as in the ideal room configuration.